Sun, Lili Zhang, Zhongjie Zhang, Ru Yu, Ye Yang, Fangying Tan, Anjiang Image_1_Molecular Disruption of Ion Transport Peptide Receptor Results in Impaired Water Homeostasis and Developmental Defects in Bombyx mori.jpg <p>Insect ion transport peptides (ITPs) are important regulators of many physiological processes and they exert their functions by interacting with their receptors (ITPRs). In the current study, we comprehensively investigated the physiological functions of ITPR in the lepidopteran model insect, the silkworm (Bombyx mori), using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) genome editing technique. Mutations in silkworm ITPR (BNGR-A2) resulted in a prolongnation of the larval stage by 3.5-day as well as failure in wing expansion of moths. The BNGR-A2 mutation accelerated food transition throughout the digestive tract, which is 1.55-fold that of wild type (WT) insects. Excretion was 1.56-fold of WT insects during the larval stage, resulting in the loss of body water content. Loss of BNGR-A2 function induced significant upregulation of nitric oxide synthase (NOS) enzyme activity and nitric oxide (NO) content, as well as downstream Ca<sup>2+</sup>/NO/cGMP signaling pathways. Key genes in insulin and ecdysone signaling pathways were also affected by BNGR-A2 disruption. Our data show that ITPR plays key roles in regulating insect water homeostasis and development.</p> Bombyx mori;ion transport peptide receptor;CRISPR/Cas9;water homeostasis;development 2020-05-20
    https://frontiersin.figshare.com/articles/figure/Image_1_Molecular_Disruption_of_Ion_Transport_Peptide_Receptor_Results_in_Impaired_Water_Homeostasis_and_Developmental_Defects_in_Bombyx_mori_jpg/12332855
10.3389/fphys.2020.00424.s001