10.3389/fonc.2020.00642.s001
Gi-Cheon Kim
Gi-Cheon
Kim
Choong-Gu Lee
Choong-Gu
Lee
Ravi Verma
Ravi
Verma
Dipayan Rudra
Dipayan
Rudra
Taemook Kim
Taemook
Kim
Keunsoo Kang
Keunsoo
Kang
Jong Hee Nam
Jong Hee
Nam
Young Kim
Young
Kim
Sin-Hyeog Im
Sin-Hyeog
Im
Ho-Keun Kwon
Ho-Keun
Kwon
Data_Sheet_1_ETS1 Suppresses Tumorigenesis of Human Breast Cancer via Trans-Activation of Canonical Tumor Suppressor Genes.pdf
Frontiers
2020
ETS1
breast cancer
tumor suppressor
DNA methylation
regulatory elements
2020-05-14 04:07:15
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_ETS1_Suppresses_Tumorigenesis_of_Human_Breast_Cancer_via_Trans-Activation_of_Canonical_Tumor_Suppressor_Genes_pdf/12300005
<p>ETS1 has shown dichotomous roles as an oncogene and a tumor suppressor gene in diverse cancers, but its functionality in breast cancer tumorigenesis still remains unclear. We utilized the Cancer Genome Atlas (TCGA) database to analyze comprehensive functions of ETS1 in human breast cancer (BRCA) patients by investigating its expression patterns and methylation status in relation to clinical prognosis. ETS1 expression was significantly diminished by hyper-methylation of the ETS1 promoter region in specimens from BRCA patients compared to a healthy control group. Moreover, ETS1<sup>high</sup> BRCA patients showed better prognosis and longer survival compared to ETS1<sup>low</sup> BRCA patients. Consistent with clinical evidence, comparative transcriptome analysis combined with CRISPR/Cas9 or shRNA based perturbation of ETS1 expression revealed direct as well as indirect mechanisms of ETS1 that hinder tumorigenesis of BRCA cells. Taken together, our study enlightens a novel function of ETS1 as a tumor suppressor in breast cancer cells.</p>