10.3389/fbioe.2020.00328.s001
Subramaniyan Manibalan
Subramaniyan
Manibalan
Ayyachamy Shobana
Ayyachamy
Shobana
Manickam Kiruthika
Manickam
Kiruthika
Anant Achary
Anant
Achary
Madasamy Swathi
Madasamy
Swathi
Renganathan Venkatalakshmi
Renganathan
Venkatalakshmi
Kandasamy Thirukumaran
Kandasamy
Thirukumaran
K. Suhasini
K.
Suhasini
Sharon Roopathy
Sharon
Roopathy
Image_1_Protein Network Studies on PCOS Biomarkers With S100A8, Druggability Assessment, and RNA Aptamer Designing to Control Its Cyst Migration Effect.pdf
Frontiers
2020
network analysis
druggability
RNA aptamer
lim method
pcos targets
protein network
2020-05-13 04:06:59
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Protein_Network_Studies_on_PCOS_Biomarkers_With_S100A8_Druggability_Assessment_and_RNA_Aptamer_Designing_to_Control_Its_Cyst_Migration_Effect_pdf/12292121
<p>The prevalence of polycystic ovary syndrome (PCOS) has been gradually increasing among adult females worldwide. Laparoscopy drilling on ovary is the only available temporary solution with a high incidence of reoccurrence. S100A8 with S100A9 complex is believed to facilitate the cyst migration in PCOS condition. The high evident protein interaction network studies between PCOS biomarkers, cancer invasion markers, and the interactors of S100A8 confirm that this protein has strong interaction with other selective PCOS biomarkers, which may be associative in the immature cyst invasion process. Through the network studies, intensive structural and pathway analysis, S100A8 is identified as a targetable protein. In this research, the non-SELEX in silico method is adapted to construct RNA Library based on the consensus DNA sequence of Glucocorticoid Response Element (GRE) and screened the best nucleotide fragments which are bound within the active sites of the target protein. Selected sequences are joined as a single strand and screened the one which competitively binds with minimal energy. In vitro follow-up of this computational research, the designed RNA aptamer was used to infect the MCF7 cell line through Lipofectamine 2000 mediated delivery to study the anti-cell migration effect. Wound Scratch assay confirms that the synthesized 18-mer oligo has significant inhibition activity toward tumor cell migration at the cellular level.</p>