10.3389/fchem.2020.00396.s001 Jing-Jing Du Jing-Jing Du Lian Zhang Lian Zhang Xiao-Fei Gao Xiao-Fei Gao Hui Sun Hui Sun Jun Guo Jun Guo Data_Sheet_1_Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides.PDF Frontiers 2020 N-linked glycopeptide glycosylation selenoester aminolysis chemical synthesis 2020-05-05 05:26:27 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Peptidyl_-Asp_Selenoesters_Enable_Efficient_Synthesis_of_N-Linked_Glycopeptides_PDF/12247970 <p>Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field. Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation. This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.</p>