10.3389/fphar.2020.00367.s003
Na Zhu
Na
Zhu
Jiang Li
Jiang
Li
Yongli Li
Yongli
Li
Yuwei Zhang
Yuwei
Zhang
Qiubo Du
Qiubo
Du
Peiyuan Hao
Peiyuan
Hao
Jinying Li
Jinying
Li
Xueming Cao
Xueming
Cao
Li Li
Li
Li
Table_1_Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1α/BNIP3 Pathway.doc
Frontiers
2020
berberine
myocardial ischemia injury
myocardial reperfusion injury
mitophagy
HIF-1α/BNIP3 pathway
2020-03-27 14:34:15
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_Berberine_Protects_Against_Simulated_Ischemia_Reperfusion_Injury-Induced_H9C2_Cardiomyocytes_Apoptosis_In_Vitro_and_Myocardial_Ischemia_Reperfusion-Induced_Apoptosis_In_Vivo_by_Regulating_the_Mitophagy-Mediated_HIF-1_BNIP3_Pathway_d/12040350
<p>Berberine (BBR) has a variety of pharmacological activities and is widely used in Asian countries. However, the clinical application of BBR still lacks scientific basis, what protective mechanism of BBR against myocardial ischemia-reperfusion injury (MIRI). In vitro experiments, BBR pretreatment regulated autophagy-related protein expression, induced cell proliferation and autophagosome formation, and reduced the mitochondrial membrane potential (ΔΨm) increase in H9C2 cells. In vivo experiments, BBR reduced the myocardial infarct size, decreased cardiomyocyte apoptosis, and markedly decreased myocardial enzyme (CK-MB, LDH, and AST) activity-induced I/R. In addition, upon BNIP3 knockdown, the regulatory effects of BBR on the above indicators were weakened both in H9C2 cells and in vivo. Luciferase reporter and ChIP assays indicated that BBR mediated BNIP3 expression by enhancing the binding of HIF-1α to the BNIP3 promoter. BBR protects against myocardial I/R injury by inducing cardiomyocytes proliferation, inhibiting cardiomyocytes apoptosis, and inducing the mitophagy-mediated HIF-1α/BNIP3 pathway. Thus, BBR may serve as a novel therapeutic drug for myocardial I/R injury.</p>