10.3389/fimmu.2020.00431.s001
Gunnveig Grødeland
Gunnveig
Grødeland
Marta Baranowska-Hustad
Marta
Baranowska-Hustad
Justin Abadejos
Justin
Abadejos
Tanya R. Blane
Tanya R.
Blane
John Teijaro
John
Teijaro
David Nemazee
David
Nemazee
Bjarne Bogen
Bjarne
Bogen
Data_Sheet_1_Induction of Cross-Reactive and Protective Antibody Responses After DNA Vaccination With MHCII-Targeted Stem Domain From Influenza Hemagglutinin.PDF
Frontiers
2020
DNA vaccine
hemagglutinin (HA)
MHCII
APC-targeted antigen
stem domain
VH1-69
2020-03-25 17:06:53
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Induction_of_Cross-Reactive_and_Protective_Antibody_Responses_After_DNA_Vaccination_With_MHCII-Targeted_Stem_Domain_From_Influenza_Hemagglutinin_PDF/12030789
<p>Novel and more broadly protective vaccines against influenza are needed to efficiently meet antigenic drift and shift. Relevant to this end, the stem domain of hemagglutinin (HA) is highly conserved, and antibodies specific for epitopes located to the stem have been demonstrated to be able to confer broad protection against various influenza subtypes. However, a remaining challenge is to induce antibodies against the poorly immunogenic stem by vaccination strategies that can be scaled up for prophylactic vaccination of the general population. Here, we have developed DNA vaccines where the conserved stem domain of HA from influenza A/PR/8/34 (H1N1) and A/Shanghai/2/2013 (H7N9) was targeted toward MHC class II molecules on antigen-presenting cells (APC) for increased immunogenicity. Each of these vaccines induced antibodies that cross-reacted with other subtypes in the corresponding phylogenetic influenza groups. Importantly, when mixing the MHCII-targeted stem domains from H1N1 and H7N9 influenza viruses into one vaccine bolus, we observed broad protection against candidate stains from both phylogenetic groups 1 and 2.</p>