10.3389/fpls.2020.00008.s002
Evangelos Karagiannis
Evangelos
Karagiannis
Georgia Tanou
Georgia
Tanou
Federico Scossa
Federico
Scossa
Martina Samiotaki
Martina
Samiotaki
Michail Michailidis
Michail
Michailidis
Maria Manioudaki
Maria
Manioudaki
François Laurens
François
Laurens
Dominique Job
Dominique
Job
Alisdair R. Fernie
Alisdair R.
Fernie
Mathilde Orsel
Mathilde
Orsel
Athanassios Molassiotis
Athanassios
Molassiotis
Presentation_2_Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald.pptx
Frontiers
2020
apple fruit
ethylene inhibition
glutathione S-transferases
metabolites
ozone
proteomics
ripening
superficial scald
2020-02-13 04:17:31
Presentation
https://frontiersin.figshare.com/articles/presentation/Presentation_2_Systems-Based_Approaches_to_Unravel_Networks_and_Individual_Elements_Involved_in_Apple_Superficial_Scald_pptx/11845785
<p>Superficial scald is a major physiological disorder in apple fruit that is induced by cold storage and is mainly expressed as brown necrotic patches on peel tissue. However, a global view of the gene-protein-metabolite interactome underlying scald prevention/sensitivity is currently missing. Herein, we have found for the first time that cold storage in an atmosphere enriched with ozone (O<sub>3</sub>) induced scald symptoms in ‘Granny Smith’ apple fruits during subsequent ripening at room temperature. In contrast, treatment with the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) reversed this O<sub>3</sub>-induced scald effect. Amino acids, including branched-chain amino acids, were the most strongly induced metabolites in peel tissue of 1-MCP treated fruits. Proteins involved in oxidative stress and protein trafficking were differentially accumulated prior to and during scald development. Genes involved in photosynthesis, flavonoid biosynthesis and ethylene signaling displayed significant alterations in response to 1-MCP and O<sub>3</sub>. Analysis of regulatory module networks identified putative transcription factors (TFs) that could be involved in scald. Subsequently, a transcriptional network of the genes-proteins-metabolites and the connected TFs was constructed. This approach enabled identification of several genes coregulated by TFs, notably encoding glutathione S-transferase (GST) protein(s) with distinct signatures following 1-MCP and O<sub>3</sub> treatments. Overall, this study is an important contribution to future functional studies and breeding programs for this fruit, aiding to the development of improved apple cultivars to superficial scald.</p>