10.3389/fgene.2019.01340.s001 Boping Tang Boping Tang Zhongkai Wang Zhongkai Wang Qiuning Liu Qiuning Liu Huabin Zhang Huabin Zhang Senhao Jiang Senhao Jiang Xinzheng Li Xinzheng Li Zhengfei Wang Zhengfei Wang Yue Sun Yue Sun Zhongli Sha Zhongli Sha Hui Jiang Hui Jiang Xugan Wu Xugan Wu Yandong Ren Yandong Ren Haorong Li Haorong Li Fujun Xuan Fujun Xuan Baoming Ge Baoming Ge Wei Jiang Wei Jiang Shusheng She Shusheng She Hongying Sun Hongying Sun Qiang Qiu Qiang Qiu Wen Wang Wen Wang Qun Wang Qun Wang Gaofeng Qiu Gaofeng Qiu Daizhen Zhang Daizhen Zhang Yongxin Li Yongxin Li DataSheet_1_High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution.docx Frontiers 2020 Eriocheir japonica sinensis evolution nanopore crab genome assembly and annotation 2020-01-17 14:02:08 Dataset https://frontiersin.figshare.com/articles/dataset/DataSheet_1_High-Quality_Genome_Assembly_of_Eriocheir_japonica_sinensis_Reveals_Its_Unique_Genome_Evolution_docx/11637471 <p>As an important freshwater aquaculture species in China, the Chinese mitten crab (Eriocheir japonica sinensis) has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development and genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of E. j.sinensis genome was approximately 1.27 Gb, which is close to the estimated size (1.19 Gb). Furthermore, based on assessment using Benchmarking Universal Single-Copy Orthologs (BUSCO) (Simao et al., 2015), 94.00% of the expected eukaryotic genes were completely present in the genome assembly. In addition, repetitive sequences accounted for ~61.42% of the assembled genome, and 22,619 protein-coding genes were annotated. Comparative genomics analysis demonstrated that the Chinese mitten crab diverged from Penaeus vannamei ~373.6 million years ago, with a faster evolution rate than shrimp. We anticipate that the annotated high-quality genome of E. j. sinensis will promote research on its basic development and evolution and make substantial contributions to comparative genomic analyses of crustaceans.</p>