Data_Sheet_1_Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets.pdf Yanling Hu Chaoliang Tan Xin Lin Zhuangchai Lai Xiao Zhang Qipeng Lu Ning Feng Dongliang Yang Lixing Weng 10.3389/fchem.2019.00844.s001 https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Exonuclease_III-Regulated_Target_Cyclic_Amplification-Based_Single_Nucleotide_Polymorphism_Detection_Using_Ultrathin_Ternary_Chalcogenide_Nanosheets_pdf/11341949 <p>Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research.</p> 2019-12-09 14:27:41 ternary chalcogenide nanosheets single nucleotide polymorphisms two-dimensional nanomaterials fluorescent detection sensor