10.3389/fmicb.2019.02829.s007 Amélie de Vallée Amélie de Vallée Pascal Bally Pascal Bally Christophe Bruel Christophe Bruel Lucie Chandat Lucie Chandat Mathias Choquer Mathias Choquer Cindy Dieryckx Cindy Dieryckx Jean William Dupuy Jean William Dupuy Sophie Kaiser Sophie Kaiser Marie-Pascale Latorse Marie-Pascale Latorse Elise Loisel Elise Loisel Géraldine Mey Géraldine Mey Guillaume Morgant Guillaume Morgant Christine Rascle Christine Rascle Julia Schumacher Julia Schumacher Adeline Simon Adeline Simon Eytham Souibgui Eytham Souibgui Muriel Viaud Muriel Viaud François Villalba François Villalba Nathalie Poussereau Nathalie Poussereau Table_6_A Similar Secretome Disturbance as a Hallmark of Non-pathogenic Botrytis cinerea ATMT-Mutants?.XLSX Frontiers 2019 Botrytis cinerea Agrobacterium tumefaciens-mediated transformation pathogenicity secretomics transcriptomics 2019-12-06 04:11:15 Dataset https://frontiersin.figshare.com/articles/dataset/Table_6_A_Similar_Secretome_Disturbance_as_a_Hallmark_of_Non-pathogenic_Botrytis_cinerea_ATMT-Mutants_XLSX/11328725 <p>The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated by random insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation (ATMT). Twelve mutants exhibiting total loss of virulence toward different host plants were chosen for detailed analyses. Their molecular characterization revealed a single T-DNA insertion in different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably deficiencies in the secretion of proteases and hemicellulases, were observed and validated by biochemical tests. They were also a hallmark of the remaining eight non-pathogenic strains, suggesting the importance of these secreted proteins in the infection process. In the twelve non-pathogenic mutants, the differentiation of infection cushions was also impaired, suggesting a link between the penetration structures and the secretion of proteins involved in the virulence of the pathogen.</p>