10.3389/fmicb.2019.02829.s007
Amélie de Vallée
Amélie
de Vallée
Pascal Bally
Pascal
Bally
Christophe Bruel
Christophe
Bruel
Lucie Chandat
Lucie
Chandat
Mathias Choquer
Mathias
Choquer
Cindy Dieryckx
Cindy
Dieryckx
Jean William Dupuy
Jean William
Dupuy
Sophie Kaiser
Sophie
Kaiser
Marie-Pascale Latorse
Marie-Pascale
Latorse
Elise Loisel
Elise
Loisel
Géraldine Mey
Géraldine
Mey
Guillaume Morgant
Guillaume
Morgant
Christine Rascle
Christine
Rascle
Julia Schumacher
Julia
Schumacher
Adeline Simon
Adeline
Simon
Eytham Souibgui
Eytham
Souibgui
Muriel Viaud
Muriel
Viaud
François Villalba
François
Villalba
Nathalie Poussereau
Nathalie
Poussereau
Table_6_A Similar Secretome Disturbance as a Hallmark of Non-pathogenic Botrytis cinerea ATMT-Mutants?.XLSX
Frontiers
2019
Botrytis cinerea
Agrobacterium tumefaciens-mediated transformation
pathogenicity
secretomics
transcriptomics
2019-12-06 04:11:15
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_6_A_Similar_Secretome_Disturbance_as_a_Hallmark_of_Non-pathogenic_Botrytis_cinerea_ATMT-Mutants_XLSX/11328725
<p>The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated by random insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation (ATMT). Twelve mutants exhibiting total loss of virulence toward different host plants were chosen for detailed analyses. Their molecular characterization revealed a single T-DNA insertion in different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably deficiencies in the secretion of proteases and hemicellulases, were observed and validated by biochemical tests. They were also a hallmark of the remaining eight non-pathogenic strains, suggesting the importance of these secreted proteins in the infection process. In the twelve non-pathogenic mutants, the differentiation of infection cushions was also impaired, suggesting a link between the penetration structures and the secretion of proteins involved in the virulence of the pathogen.</p>