10.3389/fmicb.2019.02796.s004
Manuela R. Pausan
Manuela R.
Pausan
Cintia Csorba
Cintia
Csorba
Georg Singer
Georg
Singer
Holger Till
Holger
Till
Veronika Schöpf
Veronika
Schöpf
Elisabeth Santigli
Elisabeth
Santigli
Barbara Klug
Barbara
Klug
Christoph Högenauer
Christoph
Högenauer
Marcus Blohs
Marcus
Blohs
Christine Moissl-Eichinger
Christine
Moissl-Eichinger
Table_3_Exploring the Archaeome: Detection of Archaeal Signatures in the Human Body.xlsx
Frontiers
2019
human archaeome
amplicon sequencing
human body
detection
methodology
2019-12-05 04:07:01
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_3_Exploring_the_Archaeome_Detection_of_Archaeal_Signatures_in_the_Human_Body_xlsx/11320586
<p>Due to their fundamentally different biology, archaea are consistently overlooked in conventional microbiome surveys. Using amplicon sequencing, we evaluated methodological set-ups to detect archaea in samples from five different body sites: respiratory tract (nasal cavity), digestive tract (mouth, appendix, and stool) and skin. With optimized protocols, the detection of archaeal ribosomal sequence variants (RSVs) was increased from one (found in currently used, so-called “universal” approach) to 81 RSVs in a representative sample set. The results from this extensive primer-evaluation led to the identification of the primer pair combination 344f-1041R/519F-806R which performed superior for the analysis of the archaeome of gastrointestinal tract, oral cavity and skin. The proposed protocol might not only prove useful for analyzing the human archaeome in more detail but could also be used for other holobiont samples.</p>