10.3389/fmicb.2019.02796.s003 Manuela R. Pausan Manuela R. Pausan Cintia Csorba Cintia Csorba Georg Singer Georg Singer Holger Till Holger Till Veronika Schöpf Veronika Schöpf Elisabeth Santigli Elisabeth Santigli Barbara Klug Barbara Klug Christoph Högenauer Christoph Högenauer Marcus Blohs Marcus Blohs Christine Moissl-Eichinger Christine Moissl-Eichinger Table_2_Exploring the Archaeome: Detection of Archaeal Signatures in the Human Body.xlsx Frontiers 2019 human archaeome amplicon sequencing human body detection methodology 2019-12-05 04:07:01 Dataset https://frontiersin.figshare.com/articles/dataset/Table_2_Exploring_the_Archaeome_Detection_of_Archaeal_Signatures_in_the_Human_Body_xlsx/11320583 <p>Due to their fundamentally different biology, archaea are consistently overlooked in conventional microbiome surveys. Using amplicon sequencing, we evaluated methodological set-ups to detect archaea in samples from five different body sites: respiratory tract (nasal cavity), digestive tract (mouth, appendix, and stool) and skin. With optimized protocols, the detection of archaeal ribosomal sequence variants (RSVs) was increased from one (found in currently used, so-called “universal” approach) to 81 RSVs in a representative sample set. The results from this extensive primer-evaluation led to the identification of the primer pair combination 344f-1041R/519F-806R which performed superior for the analysis of the archaeome of gastrointestinal tract, oral cavity and skin. The proposed protocol might not only prove useful for analyzing the human archaeome in more detail but could also be used for other holobiont samples.</p>