Table_1_Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities.xlsx
Juliana Minardi Nascimento
VerĂ´nica M. Saia-Cereda
Rafaela C. Sartore
Rodrigo Madeiro da Costa
Clarissa S. Schitine
Hercules Rezende Freitas
Michael Murgu
Ricardo A. de Melo Reis
Stevens K. Rehen
Daniel Martins-de-Souza
10.3389/fcell.2019.00303.s002
https://frontiersin.figshare.com/articles/dataset/Table_1_Human_Cerebral_Organoids_and_Fetal_Brain_Tissue_Share_Proteomic_Similarities_xlsx/11294870
<p>The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases.</p>
2019-11-28 11:24:02
brain organoids
neural cells
proteomics
oligodendrocyte progenitors
stem cells